THE EFFECT OF SENNA ALATA LEAF EXTRACT ON MIGRATIONAND INVASION OF CHONDROSARCOMA CANCER CELL LINE

Abstract

Chondrosarcoma, the second most common malignant bone tumor, originates from chondrocytes and is caused by abnormal proliferation. The major cause of death among cancer patients is due to the process of metastasis. Senna alata (SA) or candle bush is a traditional medicinal plant with a wide range of biological activities. In this study, cell viability was determined by MTT assay. The inhibitory effects of the SA extract on cancer metastasis were determined by cell migration, cell invasion, and cell adhesion using wound healing assay, Transwell assay, and cell adhesion assay, respectively. The SA extract inhibited cell migration, cell invasion, and cell adhesion of SW1353 cells in a dose-dependent manner. Cell signaling protein expression was investigated by Western blot analysis indicated that MMP-2 and MMP-9 expression were downregulated, while TIMP-1 and TIMP-2 expression were upregulated in SW1353 treated cells. Moreover, SA extract also decreased the expression of NF-KB transcription factor that involved cancer metastasis leading to the reduction of cellular migration and invasion by decreasing the expression of p-ERK1/2, p-p38, and PI3k/Akt signaling proteins. These results revealed that inhibition of NF-KB, MAPK, and PI3K/Akt signaling pathway were associated with MMP-2 and MMP-9 downregulation leading to a reduction in cancer cell metastasis. Therefore, these results clearly suggest that SA may be a promising agent against human chondrosarcoma metastasis.-