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dc.contributorTHUNTAWAT AUNGULDEEen
dc.contributorทัณฑวัต อังกุลดีth
dc.contributor.advisorBenjamas Wongsatayanonen
dc.contributor.advisorเบญจมาส วงศ์สัตยนนท์th
dc.contributor.otherSrinakharinwirot University. Faculty of Medicineen
dc.date.accessioned2020-12-24T05:50:37Z-
dc.date.available2020-12-24T05:50:37Z-
dc.date.issued15/5/2020
dc.identifier.urihttp://ir-ithesis.swu.ac.th/dspace/handle/123456789/900-
dc.descriptionDOCTOR OF PHILOSOPHY (Ph.D.)en
dc.descriptionปรัชญาดุษฎีบัณฑิต (ปร.ด.)th
dc.description.abstract A DNA vaccine was developed containing a partial consensus cysteine protease B (cpb) gene (pcDNA_cpb) derived from autochthonous L. martiniquensis strains in Thailand. BALB/c mice were intramuscularly immunized three times in two-week intervals with humanized codon cpb plasmid DNA (pcDNA_cpb) or in combination with monoolein (MO) as adjuvant (pcDNA_cpb-MO). The analysis of immune response demonstrated that pcDNA_cpb and pcDNA_cpb-MO could stimulate immune responses. In addition, 3 weeks after L. martiniquensis promastigote challenge, vaccinated mice sera showed significantly increased production of Th1 response; IgG2a and IFN-g and reduced IL-10 levels than the vehicle control mice sera. IFN-g/IL10 ratio was significantly higher in both pcDNA_cpb and pcDNA_cpb-MO with significant differences from control groups indicating  potentially protective immune responses in mice. Mice were potentially protected after three weeks of being challenged with L. martiniquensis promastigote , which was demonstrated by a significantly lower parasite burden in the livers and spleens of mice quantitated by qPCR and anti-LPG binding to amastigotes using an immunohistochemical method.  Monoolein, as an adjuvant, produced a booster effect to enhance mice Th1 protective immunity. This is the first report of the potential protection of partial cysteine protease B DNA vaccine of L. martiniquensis in a mouse model.en
dc.description.abstract-th
dc.language.isoen
dc.publisherSrinakharinwirot University
dc.rightsSrinakharinwirot University
dc.subjectLeishmaniaen
dc.subjectL. martiniquensisen
dc.subjectDNA vaccineen
dc.subjectCysteine proteaseen
dc.subjectMonooleinen
dc.subjectImmunizationen
dc.subject.classificationMedicineen
dc.titleIMMUNOGENICITY OF CYSTEINE PROTEASE LEISHMANIA MARTINIQUENSIS DNA BASED VACCINE IN MICEen
dc.titleความสามารถในการกระตุ้นภูมิคุ้มกันด้วยดีเอ็นเอวัคซีนต่อซิสทีอีนโปรติเอสของ Leishmania martiniquensis ในหนูไมส์th
dc.typeDissertationen
dc.typeปริญญานิพนธ์th
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